Table 1.

¹³C labeling (ratio of ¹³C to ¹²C ions) of the indicated sugars in M. arthritidis, M. pulmonis, and M. pneumoniae when medium is supplemented with ¹³C-glucose or ¹³C-starch.

		M. arthritidis			M. pulmonis			M. pneumoniae
	Ion ratios	$\frac{134}{133}$	$\frac{206}{204}$	$\frac{220}{217}$	$\frac{134}{133}$	$\frac{206}{204}$	$\frac{220}{217}$	$\frac{134}{133}$	$\frac{206}{204}$	$\frac{220}{217}$
13C-glucose supplement	Rhamnose	1a	1	1	2	1	3b	2	1	7b
	Glucose	1	1	2	1	1	1	1	1	1
	Mannose	1	1	1	1	1	1	1	1	1
	Ribose	1	1	1	4	6	17	17	6	17
	Galactose	NAc	NA	NA	1	1	1	1	1	1
13C-starch supplement	Rhamnose	9	290	600	7	25	467	7	21	43
	Glucose	27	2,300	16,000	26	820	6,000	26	1,100	8,300
	Mannose	2	3	7	1	1	1	1	1	2
	Ribosed	1	2	1	2	2	3	2	5	2
	Galactose	NA	NA	NA	5	5	13	5	27	7

^aEach ion ratio for the indicated sugar was divided by the ion ratio of the ¹²C standard to normalize. For both ¹²C glucose and ¹²C starch, the ratio of 134 to 133 ions, the ratio of 206 to 204 ions, and the ratio of 220 to 217 ions was 0.1, 0.1, and 0.03, respectively. For comparison, the ratio of 134 to 133 ions, 206 to 204 ions, and 220 to 217 ions was 2.7, 220, and 530, respectively, for ¹³C glucose. The ratio of 134 to 133 ions, 206 to 204 ions, and 220 to 217 ions was 2.9, 160, and 300, respectively, for ¹³C starch.

^bApparent increase in ion ratio attributed to labeled ribose (major peak) overlapping the major rhamnose peak in the chromatogram.

^cNA, Not applicable, galactose not detected in this species.

^dThere is no issue of labeled rhamnose overlapping the ribose peak because the ribose minor peak was used for this calculation.