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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: Nat Immunol. 2012 Feb 12;13(3):223–228. doi: 10.1038/ni.2236

Figure 3.

Figure 3

SAMHD1 reduces the intracellular dNTP pool and is counteracted by human and rhesus but not mouse SAMHD1. U937 cells were transduced with a control lentiviral vector (pLenti), or with a vector that expressed human SAMHD1 (hu high and hu mod), human HD206-7AA SAMHD1 (hu HD/AA), rhesus macaque SAMHD1(rh) or the two mouse SAMHD1 isoforms (mu iso1 and mu iso2). Two independent control cell lines were established (pLenti#1 and pLenti#2) in parallel with human SAMHD1 cell lines that expressed at high or moderate levels (hu high and hu mod). (a) The cells were either untreated (left) or PMA-treated (right) and SAMHD1 expression level was determined by immunoblot analysis. (b) dNTP levels were quantified in the untreated and PMA-treated U937 cells. (c) The PMA-treated U937 cell lines were infected with Δvpx or wild-type SIVmac239-HSA reporter viruses. The infected cells were stained with anti-murine CD24 monoclonal antibody and the infected cells were quantified by flow cytometry. (d) The cell lines were infected with HIV-1 luciferase reporter virus and luciferase activity was determined after two days.