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. Author manuscript; available in PMC: 2014 Jun 19.
Published in final edited form as: Cell Immunol. 2013 Jun 19;283(0):51–60. doi: 10.1016/j.cellimm.2013.06.008

Fig. 4.

Fig. 4

TAMs exhibit a more immature phenotype than T-PEMs and do not express the classical MDSCs phenotype. (A) Flow cytometric analysis of CD11b, F4/80, CD68, CD115, and Gr-1 in resting N-PEMs, T-PEMs and TAMs (MFI values). (B) Western blot analysis shows constitutive expression of iNOS, arginase and MMP9 by N-PEMs, T-PEMs and TAMs. (C) iNOS activity was detected as nitrite concentration levels in 48-h supernatants of constitutive or LPS-stimulated N-PEMs, T-PEMs and TAMs, and (D) Arginase activity measured as urea concentration levels in 48-h supernatants of constitutive and LPS-activated N-PEMs, T-PEMs and TAMs. Figures represent one of three different experiments with similar results. MFI, Mean fluorescence intensity.