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. Author manuscript; available in PMC: 2013 Sep 12.
Published in final edited form as: J Biol Chem. 2007 Dec 19;283(9):5611–5621. doi: 10.1074/jbc.M708885200

FIGURE 1. RT-PCR analysis of LRAT expression in wild-type embryos, yolk sacs, and placentas at different stages of development.

FIGURE 1

Wild-type females were sacrificed at different times during gestation from 6.5 to 14.5 dpc. From 6.5 to 9.5 dpc, the whole embryo, including maternal, intraembryonic, and extraembryonic tissues, was used for RNA extraction. At 10.5, 12.5, and 14.5 dpc, embryo, placenta, and yolk sac were dissected separately, and RNA was extracted from each. Liver from wild-type (wt) and LRAT−/− mice(−/−) were used as positive and negative controls, respectively. M, 100-bp molecular weight marker; b, blank; -RT, sample minus reverse transcription reaction. The molecular weight of the marker bands is indicated on the left. Expected molecular weights of the products: LRAT, 610 bp; actin, 556 bp.