FIG. 7.

DHE staining in coronary arteries. Production of superoxide anion radical was measured by staining with 10 μM dihydroethidium (DHE) in the isolated coronary arteries. Upper panel shows representative DHE, and the lower panel shows summary data of DHE fluorescent intensity (in arbitrary units) in the coronary arteries. The signal of DHE was potent with SMP30 deficiency, which was attenuated with apocynin (0.3 mM). The values were expressed as means±S.E.M. *p<0.01 vs. without any treatment in WT mice, ^#p<0.01, vs. without treatment in SMP30 KO mice, n=10, each.