Figure 4.

SRPK inhibition dose dependently inhibits choroidal angiogenesis. (A) Twenty-two C57/B6 mice were subjected to laser photocoagulation (IRIDEX Oculight GLX λ, 810 nm, 250 mV, 0.1 second, 75 μm, 4 lesions/eye). On day 0 and day 7 mice received i.o. injection of SRPIN340 (5, 3, 1, 0.5, 0.1 ng/μL, 2 μL) or saline in the control eye. SRPIN340 dose-dependently inhibited laser-induced neovascular growth compared with saline controls (P < 0.01, two-way ANOVA) reaching statistical significance at 5 ng/μL (P < 0.01) with an EC₅₀ of 0.32 ng/μL, total injection of 1.28 ng. Scale bar: 50 μm. (B) Twenty nanograms of SRPIN340 was injected intravitreally into the left and right eyes of 10 CD-1 mice. Two mice were culled at each of the following time points; 1, 4, 8, 24, and 48 hours, eyes were removed, tissue homogenized, proteins precipitated out, and sample lysate analyzed by mass spectrometry. SRPIN340 expression in the eye tissue decreased over time with a sustained half-life of 22 hours (one-phase exponential decay, prism).