Figure 8. Selamectin decreases proliferation and increases the expression of proneural and lineage-associated transcription factors.
(A) Representative fields show the BrdU incorporation on Day 11 after cells were treated with selamectin (right panel) or DMSO (left panel) for 4 days. Significantly fewer BrdU+ cells were detected in the selamectin-treated group. (B) Quantification as the percentage of BrdU+ cells among total cells (t-test, n = 4, p=0.008). (C) qRT-PCR detects increased expression of proneural (Ascl1, NeurD) and lineage-associated transcription factors (Lmx1a, Lmx1b and Nurr1) in selamectin-treated group. β-actin was used as an input control and data was normalized to expression level on Day 8. (D) Representative fields show the TUNEL staining on Day 14 in cell cultures treated with DMSO or selamectin, with NeuN staining as a control to confirm selamectin efficacy in this experiment. (E) Quantification shows significant difference in NeuN% (p<0.001) and no significant difference in TUNEL% (p=0.058) (t-test, n = 4). Scale bar, 10 μm.