FIGURE 2.

Effect of LXR activation on protein expression in Caco-2 monolayers. Monolayers were preincubated overnight with T0901317 or with the equivalent volume of ethanol vehicle. Mixed micelles providing 10 μmol/L α-, γ- and δ-TOH were then added apically. At 24 h, cells were collected and ABCA1 protein (A) and SR-BI protein (C) were determined by Western blot. (B) After overnight treatment with T, cells were administered normal media (FBS) or micelles (mic) and ABCG1 protein was assesed by western blot. ABC, ATP-binding cassette; C, control; SR-BI, scavenger receptor class B member I; T, T0901317.