FIGURE 7.

Role of SR-BI in basal tocopherol accumulation from Caco-2 monolayers. Monolayers were treated with BMS201038 and mixed micelles as in Fig. 1. Anti-SR-BI was added basolaterally where indicated one hour prior to addition of mixed micelles. (A) Basal accumulation of TOH after 24 h, in the presence or absence of anti-SR-BI (1:1000). (B) Basal accumulation of TOH after 24 h, when HDL (6 μg/mL) was included in the basolateral compartment in the presence or absence of anti-SR-BI as indicated. Results are expressed as means ± SDs, n = 6 independent observations. *, P < 0.005 vs. control; x, P < 0.05 vs. HDL. aSR-BI, anti-scavenger receptor class B member I (SR-BI) blocking antibody against the extracellular domain of SR-BI; chol, unesterified cholesterol; HDL, high density lipoprotein; TOH, tocopherol.