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Dronpa dark-to-fluorescent photo-activation (top). After converting Dronpa to the “dark” state in figure 2 and 3 the same sample is used for measuring reversible photo-conversion. A 405nm laser is used for converting the same three ROIs previously activated. The laser powers are respectively 0.02μW, 0.04uW and 0.35uW. 60 frames were acquired. For simplicity only 6 frames are shown (bottom) with the respective color selection indicated in the spectral phasor plot.
