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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Methods Appl Fluoresc. 2013;1(3):035001. doi: 10.1088/2050-6120/1/3/035001

Figure 5.

Figure 5

Kaede green to red photo-activation second harmonic spectral phasor analysis (top). Three ROIs are selected on the bead (53×48 pixels). Laser power at 405nm is set respectively at 0.04μW (left ROI), 1.33 μW (right ROI) and 2.69 μW (bottom ROI) for the ROIs. Zoom is set at 3.1 with a frame size of 43.06umx43.06um. 100 frames are acquired using 488nm and 561nm wavelength. Green cursor on spectral phasor plot (top) is used to select the green state, red cursor for red state. Blue selection outlines the linear combination between green and red in the same pixel. In the image, pixels are colored according to the cursor color. We show 6 out of 100 frames acquired.