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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Ther Deliv. 2013 Feb;4(2):191–202. doi: 10.4155/tde.12.151

Figure 3. Representative overlays of fluorescence and Nomarski images of (A) non-, (B) latrunculin B- and (C) nocodazole-treated cells.

Figure 3

A qualitative co-localization was obtained simply overlaying pixel-by-pixel different channels, pseudo-colored using red and green look-up tables. We used green for LysoSensor-labeled lysosomes while red for Cy3-labeled lipoplexes. The presence of the two fluorescent labels in the same pixels (i.e., co-localization) produces yellow hotspots (indicated by white arrows).

LAT: Latrunculin B; NCZ: Nocodazole; NT: Not treated.