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. 2013 Sep 12;9(9):e1003626. doi: 10.1371/journal.ppat.1003626

Figure 2. Deletion of sX13 derogates virulence gene expression.

Figure 2

(A) Xcv strains 85-10 (wt) and the sX13 deletion mutant (ΔsX13) carrying the empty vector (pB) or the sX13 expression construct (psX13) and strains additionally expressing HrpG* (phrpG*) were incubated for 3.5 hours in hrp-gene inducing medium XVM2. Total protein extracts were analyzed by immunoblotting using antibodies directed against HrpF, HrcN, HrcJ and GroEL. The experiment was repeated twice with similar results. (B) Xcv 85-10 (wt), ΔsX13 and ΔsX13+sX13 ch and strains additionally expressing HrpG* were incubated for 3.5 hours in hrp-gene inducing medium XVM2. Total protein extracts were analyzed by immunoblotting using antibodies directed against HrcJ and GroEL. The experiment was repeated twice with similar results. (C) Indicated genes were analyzed by qRT-PCR using RNA from cultures described in (B). The amount of each RNA in Xcv 85-10 was set to 1. Data points and error bars represent mean values and standard deviations obtained with three independent biological samples. Asterisks indicate statistically significant differences compared to wt (t-test; P<0.03).