Figure 3. sX13 accumulation is altered under stress conditions in Xcv 85-10.

(A) Northern blot analysis of sX13. Exponential phase cultures of NYG-grown Xcv 85-10 were transferred to NYG medium or MMA containing the indicated additives or lacking a nitrogen or carbon source (ΔN; ΔC). Cultures were shaken for three hours at 30°C unless otherwise indicated. 5S rRNA was probed as loading control. (B) sX13 and selected sX13-regulated genes (see Table 1) were analyzed by qRT-PCR using RNA from Xcv 85-10 (wt) cultures shown in (A) and NYG-grown ΔsX13. Bars represent fold-changes (log₁₀) of mRNA amounts compared to Xcv 85-10 grown in NYG at 30°C. Experiments were performed twice with similar results.