Figure 4. qRT-PCR analysis of sX13-regulated genes.

Selected sX13-regulated genes (see Table 1) were analyzed by qRT-PCR using RNA from NYG- and MMA-grown Xcv strains 85-10 (wt) and ΔsX13. The amount of each mRNA in the wt was set to 1. Bars represent fold-changes of mRNA amounts in strain ΔsX13 compared to 85-10 on a logarithmic scale (log₁₀). Data points and error bars represent mean values and standard deviations obtained with at least three independent biological samples. Asterisks denote statistically significant differences (t-test; P<0.05). Dashed lines indicate a 1.5-fold change. Transcripts not detected in the microarray analyses are marked with ‘a’.