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. 2013 Sep 12;9(9):e1003609. doi: 10.1371/journal.ppat.1003609

Figure 1. UL76 induces expression of IL-8.

Figure 1

A) Luciferase activity was measured in 293T cells co-transfected with pcDNA3 or pcDNA3HA-UL76, IL-8 luciferase reporter and β-Galactosidase plasmid at 28 h–30 h post-transfection. Luciferase activity was normalized to β-Galactosidase activity as a control for transfection efficiency. Data are expressed as means ± SD of triplicate wells from one of three similar experiments. Statistical significance is represented as ** p<0.01; *** p<0.001. B) IL-8 concentration in supernatants of 293T cells transfected with control plasmid or pcDNA3HA-UL76 was determined 48 h post-transfection by ELISA. Data are expressed as means ± SD of duplicate wells from one of three similar experiments. ** Statistically significant as compared with control vector-expressing cells (p<0.01). Expression of HA-tagged UL76 protein was confirmed by western blot (below) using an anti-HA HRP-conjugated antibody and β-actin detection was used as loading control.