Figure 7. Impact of ATM on the induction of IL-8 by HCMV.

Primary fibroblasts ATM -/- cells (GM01588) were infected with HCMV wild type at a MOI of 3. Supernatants were collected at 24 h, 48 h and 72 h post-infection and IL-8 concentration was determined by ELISA. Data are expressed as means ± SD of fold induction to mock-infected cells and are representative of three similar experiments. Cells were lysed at 72 h post-infection and viral infection was confirmed by western blot (below) using an anti-UL44 antibody for the HCMV viral protein and detection of β-actin as loading control.