Figure 5. rPAF-AH and MCP-1/CCL2 increase NO production by peritoneal macrophages stimulated with E. coli.

Peritoneal macrophages (10⁶ cells/well) were incubated with rMCP-1 (100 ng/ml), PAF-AH (0,025 mg/well) or rMCP-1+PAF-AH and stimulated with E. coli (10⁵ bact./well). After 1 hour of E. coli stimulation, the supernatant was obtained and used for nitrite determination by the Griess assay. The values are the mean±SEM from 4 wells. The data represent two different experiments. (*) Statistically significant differences when compared with E. coli group (P = 0.030).