Figure 4. miR-199 controls Ad-199T replication in vivo.

To assess replication properties of AD-199T in vivo, 1x10⁸ I.U. of Ad-199T or 1x10⁸ I.U. of Ad-Control were intra-hepatically injected into 3 days old B6D2 mice. At 72 hours after infection, livers were collected and viral DNA quantified using analytical PCR (A) and quantitative PCR as fold change copies of Adeno DNA referred to the lower level of Adenovirus DNA copies (B) The replication of Ad-199T (mice 1375, 1376) was significantly suppressed in normal liver comparing with Ad-Control (mice 1370, 1372). This evidence confirms that miR-199 could control Ad-199T replication in normal liver cells in vivo.