Figure 6. Differential replication of Ad-199T in normal liver versus tumor cells.

2x10⁶ HepLuc cells were intra-hepatically implanted in six B6D2 wild type mice 3 days after birth. After 24 hours, the animals were injected intra-hepatically with 1x10⁸ I.U. of the Ad-199T virus. Mice were sacrificed at 24h and 48h after the treatment and livers as well as tumor cells masses were collected. Genomic DNA extracted both from normal liver and tumor masses was analyzed by analytical PCR (A) and quantitative PCR as fold change copies of Adeno DNA referred to the lower level of Adenovirus DNA copies (B). The results show a significant suppression of Ad-199T replication in normal liver (NL) compared to tumors (Tumor). P-values of the comparisons are shown.