FIGURE 5:

Cells depleted of LARG undergo an Aurora-B–dependent delay in abscission kinetics. (A) Asynchronous HeLa cells were treated with siRNA for 72 h. Cells were treated with either DMSO vehicle or 2 μM ZM447439 to inhibit Aurora-B for 2 h before fixing and staining for α-tubulin (green) and DNA (blue). Arrows highlight midbody-stage intercellular bridges. Bar, 10 μm. (B) Quantification of the percentage of midbody-stage intercellular bridges for both NS si and LARG si cells in the presence of vehicle or Aurora-B inhibitor (*p ≤ 0.0012, DMSO vs. ZM447439; DMSO, NS si = 7478 cells, LARG si = 3388 cells; ZM447439, NS si = 7229 cells, LARG si = 4249 cells; across four independent experiments). (C) Percentage of midbody-stage intercellular bridges for LARG si cells normalized by subtraction of NS si cell percentages for each DMSO and ZM447439 treatment (*p = 0.0045).