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. 2013 Sep 15;24(18):2885–2893. doi: 10.1091/mbc.E12-11-0798

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© 2013 Applewhite et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — Colocalization between Shot constructs and microtubules. S2 cells cotransfected with (A) Shot A-EGFP, (B) FKBP-Shot-FRB-EGFP treated with DMSO, (C) FKBP-Shot-FRB-EGFP treated with 500 nM rapamycin, (D) Shot∆GAS2-EGFP, (E) Shot∆Rod-EGFP, (F) Shot∆EF-hand-EGFP, and (G) ShotGAS2-EGFP (green) and mCherry-tubulin (red). Scale bar: 10 μm. (H) Graph representing the mean Mander's coefficient quantifying fraction of overlap/colocalization between microtubules and EGFP-tagged Shot constructs. Error bars represent 95% confidence intervals. Statistical analysis was determined by one-way analysis of variance test with Tukey's multiple-comparison post hoc analysis performed on ∼30–40 different cells per condition. Statistical significance is denoted by asterisks (*, p < 0.05), while statistically insignificant comparisons between groups are denoted by n.s.