FIGURE 1:

Effects of depletion of RINT-1 on the distribution of ER-Golgi/ERGIC recycling proteins and the ER structure. (A) HeLa cells were mock transfected (top) or transfected with siRNA RINT-1 (1149) (second row), RINT-1 (687) (third row), ZW10 (102) (fourth row), or NAG (4160) (bottom). After 72 h, the cells were double stained with antibodies against GM130 and Rer1 or KDEL receptor. Merged images are also shown. Bar, 5 μm. (B) HeLa cells were mock transfected or transfected with siRNA RINT-1 (1149) or RINT-1 (687). After 72 h, the cells were single stained with an antibody against calnexin or Hsp47. Bar, 5 μm.