FIGURE 3:

Endosome-to-TGN transport of CTB is impaired in RINT-1–depleted cells. HeLa cells were mock transfected (top) or transfected with RINT-1 (1149) (middle) or ZW10 (102) (bottom). After 72 h, the cells were allowed to bind Alexa Fluor 594–conjugated CTB for 30 min at 4ºC (left) followed by chase at 37ºC for 45 (middle) and 90 min (right). The cells were analyzed by immunofluorescence microscopy after staining with an anti-EEA1 antibody. Bar, 5 μm.