FIGURE 5:

Overexpression of RINT-1 disrupts the localization of the COG complex but not cis-Golgi proteins. (A) The plasmid encoding FLAG–RINT-1 was transfected into HeLa cells, and at 24 h after transfection, the cells were double stained for indicated proteins (top) and FLAG–RINT-1 (bottom). Asterisks indicate FLAG–RINT-1–overexpressing cells. Bar, 5 μm. (B) Quantitation of the data shown in A. Data are the average of three independent experiments (n [cell number] ≥ 50). Error bars, SEM.