FIGURE 7:

The interactions of RINT-1 with tethers and SNAREs in the TGN and the ER. (A) At the TGN, RINT-1 serves as a link between Cog1 and syntaxin 16 through its N-terminal region (N). The Vps51-like domain of Cog1 and the SNARE domain of syntaxin 16 are responsible for the interactions with RINT-1. The COG complex model is according to Oka et al. (2005) and Ungar et al. (2005). The interaction between Cog1 and Cog4 (blue dashed line) was reported (Loh and Hong, 2004). (B) At the ER, RINT-1/Tip20 serves as a link between ZW10/Dsl1 and BNIP1/Sec20. The N-terminal regulatory region of Sec20 (shown as BNIP1N) is responsible for the interaction with Tip20 (Ren et al., 2009), and the central and C-terminal regions (shown as C) of RINT-1/Tip20 bind to BNIP1/Sec20 (Tripathi et al., 2009; this study; Supplemental Figure S7A).