Figure 2. 4HPR reduces excessive phosphorylation of MAP kinase ERK1/2 in FXR1-KO macrophages.

FXR1-WT (▪) and FXR1-KO (□) macrophage cell lines (1×10⁶ cells) were unstimulated (medium) or stimulated for 30 min with LPS (50 ng/ml). Both macrophage cell lines were also treated with 4HPR (0.62 µM) under LPS stimulation conditions. Cells were harvested and kinase phosphorylation levels were analyzed by Western Blot; Total corresponding protein levels (ERK1/2 or p38) were used to normalize differences in loading. (A) Levels of phospho-ERK1/2 and total ERK1/2 MAPK in FXR1-WT and KO cells. (B) Levels of phospho-p38 and total p38 MAPK. Results are presented as mean ± SEM of four independent experiments. Differences were analyzed using an unpaired t-test and were considered to be significant at p≤0.05. * indicates p<0.05.