Figure 5. Interactions between AA and DHA in macrophage cell lines stimulated with exogenous AA or DHA.

FXR1-WT (▪) macrophages (50×10⁶ cells) were treated with either arachidonic acid (100 nM) or docosahexaenoic acid (100 nM) for 21 h. Cell were harvested and resuspended in BHA solution. (A) AA levels in FXR1-WT macrophages; (B) DHA levels; (C) AA:DHA ratios. Results are presented as mean ± SEM of four independent experiments. Differences were analyzed by one way ANOVA followed by a Student-Newman-Keuls secondary analysis, and were considered to be significant at p≤0.05. * indicates p<0.05.