Figure 7. Biological effect of the modulation of ERK1/2 phosphorylation by AA or DHA on cytokines and chemokines gene expression.
FXR1-WT (▪) and FXR1-KO (□) macrophage cell lines (2.5×105 cells) were untreated (Medium) or treated with AA (100 nM) or DHA (100 nM) for 21 h. Cells were also stimulated with 50 ng/ml of LPS for 3 h. Cell supernatants were harvested and levels of IL-6 (A), CCL2 (B), and CCL5 (C) were analyzed by Luminex platform assay. Results are presented as mean ± SEM of three independent experiments. Differences were analyzed using an unpaired t-test and were considered to be significant at p≤0.05. * indicates p<0.05.