Skip to main content
. 2013 Sep 12;8(9):e72926. doi: 10.1371/journal.pone.0072926

Figure 3. Protective effects of MSC CM against STS toxicity in NSC-34 cells.

Figure 3

NSC-34 cells were incubated with DMEM conditioned by MSC (CM) starting 4 h before exposure to STS (0.03 µM). A: STS-induced apoptosis was attenuated best by a 50% dilution of CM, as shown by MTT assay. B: Quantification of cell death by immunocytochemical analysis similarly revealed most neuroprotection at a 10–50% dilution of MSC CM. Values represent means ± SEM, ***p<0.001, **p<0.01, *p<0.05, one-way ANOVA with Bonferroni post-test. C: Immunostainings showed a reduction of cell number due to induction of apoptosis by STS, as well as the protective effect of MSC CM. a: NSC-34 cell monoculture without any treatment. b: Reduction of ß-III tubulin positive NSC-34 cells following exposure to 0.03 µM STS. c: Increase in NSC-34 cell survival due to CM treatment. NSC-34 cells were stained by an antibody against ß-III tubulin (green). Stained nuclei of cultured cells appear in blue (DAPI). Scale bar 100 µm.