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. 2013 Sep 12;8(9):e72926. doi: 10.1371/journal.pone.0072926

Figure 7. Influence of MSC CM on astrocytic and microglial gene expression.

Figure 7

The relative mRNA expression of the pro-inflammatory cytokines TNFα and IL-6, the anti-inflammatory cytokine IL-10, the pro-inflammatory enzymes iNOS and COX2, and the neuroprotective chemokine CX3CL1 and its receptor CX3CR1 were examined in non-transgenic and SOD1G93A transgenic astrocyte cultures incubated for 28 h in 100% MSC CM or regular medium without CM and with or without LPS stimulation (500 ng/mL, added during the last 4 h) (A). The relative mRNA expression of the corresponding receptor CX3CR1 was analysed in non-transgenic and SOD1G93A transgenic microglia cultures co-cultured with or without MSC in a transwell system (B). LPS-induced upregulation of the pro-inflammatory cytokines IL-6 and TNFα and of the pro-inflammatory enzyme iNOS was significantly higher in transgenic than in non-transgenic astrocytes and was significantly attenuated by MSC CM (A). MSC CM significantly upregulated CX3CL1 expression in SOD1G93A transgenic astrocytes, while the MSC CM induced increase in CX3CL1 in non-transgenic astrocytes was not statistically significant. MSC significantly increased the expression of CX3CR1 in transgenic microglial cells, and slightly but not significantly upregulated it also in non-transgenic ones (B). Values represent means ± SEM, §§§/###/***p<0.001, #/*p<0.05, two-way ANOVA with Bonferroni post-test.