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. 2013 Sep 12;8(9):e72588. doi: 10.1371/journal.pone.0072588

Figure 2. Plain PPS-1 booster abrogates the Pnc1-TT-induced GC reaction in mice primed as neonates.

Figure 2

(A–C) Mean number of follicles and (D) ratio of GC/follicle per section in consecutive sections from spleen of mice that received booster by either s.c. (left columns) or i.n. (right columns) route with saline (open columns), PPS-1+LT-K63 (black columns), Pnc1-TT+LT-K63 (grey columns) and unvaccinated controls (stribe columns), as indicated. Spleens were removed 7 days after booster in mice primed as neonates with Pnc1-TT+LT-K63 by the same route as the booster. Half of the spleen was snap frozen, serial cryosection prepared, cutting into 7 µm sections at four levels, starting 700 µm into the tissue and the levels separated by 210 µm. Section from all 4 levels were stained with (A) anti-IgM, (B) anti-IgG, (C) PNA, and results (mean±SD) shown for each group. (D) Mean ratio of GC/follicle was calculated for every spleen at all 4 levels for individual mice and results (mean±SD) shown for each group. Statistical difference between vaccinated groups is stated in the graphs. Results in A–D are from one representative of two independent experiments (8 mice per group) showing comparable results.