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. 2013 Sep 12;8(9):e73642. doi: 10.1371/journal.pone.0073642

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© 2013 Verma et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Footnote: The lipolytic activity was determined following the method of Winkler and Stuckmann (1979) [21], using p-NP palmitate as substrate. Bars indicate the standard deviation from triplicate determinations. b) Effect of temperature on the activity of free and immobilised lipase. Footnote: The lipolytic activity was determined following the method of Winkler and Stuckmann [21], using p-NP palmitate as substrate. Bars indicate the standard deviation from triplicate determinations.