Figure 8. Reusability study of the immobilised lipase.

Footnote: The lipolytic activity was determined following the method of Winkler and Stuckmann [21], using p-NP palmitate as substrate. Bars indicate the standard deviation from triplicate determinations. Residual activity of immobilised enzyme at different cycles. 1 cycle = 100±4.2; 2 cycle = 88±3.9; 3 cycle = 84±4.0; 4 cycle = 80±2.9; 5 cycle = 70±3.0; 6 cycle = 64±4.4; 7 cycle = 62±3.9; 8 cycle = 56±4.5; 9 cycle = 54±4.8; 10 cycle = 51±4.3; 11 cycle = 49±4.2.