Table 2. Fractions of each type of secondary structure in lipase covalently immobilised to amino-functionalised MWNT.
Structure | H(r) | H(d) | S(r) | S(d) | Trn | Unrd |
Native enzyme | 0.607 | 0.265 | 0.002 | 0.002 | 0.009 | 0.116 |
Immobilised enzyme | 0.491 | 0.189 | 0.006 | 0.013 | 0.095 | 0.206 |
Abbreviations: H(r), regular α-helix; H(d), distorted α-helix; S(r), regular β sheet; s(d), distorted β sheet; Trn, Turn; Unrd, unordered. All experiments in this study were carried out in triplicate with standard deviation below 5%.