Table 3. Kinetics of free and immobilised lipase using p-NP palmitate as substrate.

Parameters	Free enzyme	Immobilised enzyme
KM (mM)	3.1	4.5
Vmax (U/mg)	20.1	22.9

Footnote: Initial rates were estimated from the extent of substrate hydrolysis during 10 min incubation at 60°C. Reaction mixtures contained 10–60 mM p-NP palmitate, free and immobilised lipase (2 U), in 2.9 mL in 50 mM Tris buffer (pH 9.0). Kinetic parameters were calculated from GraphPad software (version 6.0). All experiments in this study was carried out in triplicate with standard deviation below 5%.