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Primer MFT-F1M and MFT-A1R1 were used to amplify the complete TaMFT-A1 from three nullisomic-tetrasomic (NT) Chinese Spring (CS) lines, N3AT3B (1), N3BT3A (2), N3DT3A (3), as well as Jagger (4) and 2174 (5).
