Figure 5. Activation of the viral fusion process is required for RVFV-6 binding to RVFV Gc.

Biotin-conjugated RVFV-6, RVFV-6sc, or no peptide was pre-bound to avidin beads before the addition of RVFV-MP12. Beads were washed to remove unbound virus and treated as indicated with 1) lysis buffer and wash, 2) pH 5.2 treatment followed by lysis buffer and wash, or 3) no pH 5.2 treatment and no lysis buffer. Protein bound to the avidin beads were resolved by SDS-PAGE and probed with the anti-RVFV Gc antibody 4D4. Data represent at least 3 separate experiments.