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. 2013 Sep 12;8(9):e73869. doi: 10.1371/journal.pone.0073869

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© 2013 Wei et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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TbKIN-C and TbKIN-D were each tagged with EYFP or triple HA epitope at the C-terminus in their endogenous locus. Localization of EYFP-tagged proteins was examined directly under a fluorescence microscope, whereas localization of 3HA-tagged proteins were monitored after immunostaining the cells with FITC-conjugated anti-HA antibody. Cells were fixed with paraformaldehyde. Arrows indicate the enrichment of TbKIN-C at the posterior tip of the cells. Bars: 5 µm.