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. 2013 Sep 12;8(9):e73869. doi: 10.1371/journal.pone.0073869

Figure 8. Effect of TbKIN-C RNAi on TbKIN-D protein level and TbKIN-D RNAi on TbKIN-C protein level in the bloodstream form.

Figure 8

(A). Western blot to detect TbKIN-D protein level in TbKIN-C RNAi cells and TbKIN-C protein level in TbKIN-D RNAi cells in the bloodstream form. (B). Western blot to detect TbKIN-D protein level in TbKIN-C RNAi cells and TbKIN-C protein level in TbKIN-D RNAi cells in the procyclic form. MG132 (25 µM) was added to TbKIN-C RNAi cells (day 2) and TbKIN-D RNAi cells (day 3) and incubated for 6 hrs. (C). TbKIN-C and TbKIN-D mRNA level in TbKIN-C RNAi cells and TbKIN-D RNAi cells measured by quantitative RT-PCR in the bloodstream form.