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. 2013 Sep 12;9(9):e1003766. doi: 10.1371/journal.pgen.1003766

Figure 4. RKS1 allelic forms and expression in susceptible and resistant accessions of Arabidopsis thaliana.

Figure 4

(A) Schematic representation of resistant (Col-0) and susceptible (Kas-1) RKS1 allele polymorphisms. Sequence changes in both alleles are indicated. (B) Schematic representation of the most frequent 5′ and 3′ ends of RKS1 transcripts found by 5′ and 3′ RACE experiments in resistant (Col-0, green) and susceptible (Kas-1, red) accessions. (C) RKS1 gene expression evaluated by Q-RT-PCR in germinating seeds and in leaves, healthy or inoculated with Xcc568, from the resistant accession (Col-0, green) and the susceptible accession (Kas-1, red). Different primers are used to evaluate long (L) and long+short (L+S) transcripts (Table S2) A.U.: arbitrary units. (D) Correlation between RKS1 gene expression after infection with Xcc568 and resistance phenotype. The dashed line indicates an exponentially decreasing function fitted on the median values of the 13 types of genetic line. Numbers in brackets indicate the number of representatives of each type of transgenic line.