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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Arthritis Rheum. 2013 Jul;65(7):1843–1852. doi: 10.1002/art.37977

Figure 6. Whole reconstruction of tibial plateau showing distribution of GFP-LC3 signal in response to GlcN.

Figure 6

GFP-LC3 transgenic mice were treated with GlcN and knee joints were collected as described in Figure 5. Sagittal vibratome-cut sections were stained with anti-LC3 far red and Hoechst 33342 to label LC3 and the nuclei, respectively and then analyzed by confocal microscopy. Representative images of GFP-LC3 signal in response to GlcN treatment from cartilage knee joints are shown. A merged fluorescence image is shown in the bottom panel; GFP-LC3 (green), LC3 (red), nuclei (blue). The 4th panel (right) series represents a 3D IMARIS rendered reconstruction of the 3rd panel series, where maximum induction is observed.