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. 2013 Jul 26;288(37):26521–26532. doi: 10.1074/jbc.M113.475053

FIGURE 5.

FIGURE 5.

A, schematics of α4β2 nAChR expression with principal (+) and complementary (−) sides of the receptor interfaces are shown. The (α4)3(β2)2, (α4[D204C])3(β2)2 (1:1 mRNA injection ratio), and (α4[D204C])2(β2)3 (1:10 mRNA injection ratio) are represented with α subunits (black circles), with the D204C mutation (white dot) shown, and with β subunits (gray circles). The D204C mutation is located at the α4-α4 and β2-α4 interfaces of the (α4[D204C])3(β2)2 receptor and at the β2-α4 interfaces only on the (α4[D204C])2(β2)3 receptor. The reactive MLA-maleimide (white square) is covalently trapped with the D204C mutant residue only at the α4-α4 interface of the (α4[D204C])3(β2)2 receptor. B, shown is a structural model of the α4-α4 interface with the MLA-maleimide covalently trapped by cysteine (D204C).