TABLE 2.
ACh concentration-response curve in the presence of a fixed concentration of MLA; parameters derived from curve fitting
Error is from Student's t test two-tailed, unequal variance, logEC50 of individual experiments used for statistical comparisons.
| Receptor (ratio) | Incubation | [MLA] | I/IACh (95% CI)a | EC50 (95% CI) | nH (95% CI) | n |
|---|---|---|---|---|---|---|
| m | m | |||||
| α7(L9'T) | 3 min | 1 × 10−10 | 0.97 (0.87–1.06) | 6.6 × 10−6 (5–9 × 10−6)b,c | 1.8 (1–2.8) | 4 |
| α4β2 (1:1) | 3 min | 2 × 10−7 | 0.46 (0.35–0.58)b,d | 9.5 × 10−5 (3–30 × 10−5) | 0.8 (0.2–1.4) | 5 |
| α4β2 (1:1) | 0 min | 2 × 10−6 | 1.12 (0.99–1.26) | 6.7 × 10−4 (4.1–11 × 10−4)e,f | 0.98 (0.6–1.4) | 5 |
| α4β2 (1:1) | 3 min | 1 × 10−8 | 1.14 (1.05–1.2) | 1.9 × 10−4 (1.4–2.6 × 10−4) | 0.8 (0.6–1.0) | 4 |
| α4β2 (1:1) | 3 min | 3 × 10−8 | 0.71 (0.61–0.8)b,f | 1.0 × 10−4 (0.6–1.8 × 10−4) | 1.1 (0.5–1.6) | 3 |
| α4β2 (1:1) | 3 min | 1 × 10−6 | 0.22 (0.18–0.25)b,d | 8.2 × 10−5 (4.6–15 × 10−5)b,f | 1.5 (0.3–2.8) | 5 |
a I/IACh normalized to 10 μm ACh for α7(L9'T); 1 mm for all others.
b Compared to value without MLA application.
c p < 0.01.
d p < 0.001.
e Compared to the value without incubation.
f p < 0.05.