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Siglec-F ligands in BAL fluid. A, fractions of BAL fluid assayed by ELISA using Siglec-F-Fc (filled circles), or anti-MUC5B (filled squares). Total protein was determined by measuring absorbance at 280 nm (dotted line). No signal was observed when wells were reacted with CD22-Fc (open squares), human IgG (open triangles), or treated with sialidase before incubation with Siglec-F-Fc (open circles). Results are representative of two independent experiments.
