FIGURE 3.

Effect of Lig3 knockdown on physiological responses of the HeLa cells and DNA repair. A, effect of Lig3 knockdown on mitochondrial membrane potential as measured by JC-1 fluorescence. Val, 0.2 μm valinomycin (ionophore). B, effect of Lig3 knockdown on cellular respiration as measured by an XF-24 analyzer. Oxygen consumption is expressed in picomoles of O₂/min/μg of protein. C and D, effect of Lig3 knockdown on susceptibility to DNA-damaging agents MMS (C) and hydrogen peroxide (H₂O₂, D) as determined by clonogenic assay. Two-tailed Student's t test assuming unequal variance; n = 5 or 6. ns, not significant; *, p < 0.05; **, p < 0.01; ***, p < 0.001.