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. 2013 Jul 24;288(37):26594–26605. doi: 10.1074/jbc.M113.472977

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Lig3^−/− cells are dependent on uridine and pyruvate for their survival and growth. A, representative microscopic field views of Cre4/2941 cells exposed to media with and without uridine and pyruvate for 0 h (immediately after media change) and 24, 48, and 72 h after media change. Top panels, DMEM without uridine or pyruvate supplementation. Bottom panels, DMEM supplemented with uridine and pyruvate. Cells proliferate in media supplemented with uridine and pyruvate and die in unsupplemented media. B, growth curves of Cre4, Cre4/2941, Cre4 ρ⁰#11, and Cre4/3441/3491 in media with or without uridine and pyruvate. Note that neither Cre4 ρ⁰#11 (ρ⁰ due to EtBr treatment) nor Cre4/2941 (ρ⁰ due to Lig3 deletion) survive in media without uridine or pyruvate.