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Enzymatic activity of wild-type AguA1 and AguA2 and their mutant proteins. A, relative activity of wild-type AguA1 and its mutants (D94A/D94E, E155A/E155D, H216A/H216R, D218A/D218E, and C356A). B, enzymatic activity of wild-type AguA2 and its mutants (Y47F, C157G, F196L, N236D, and H360Q). C, enzymatic activity of wild-type AguA1 and its mutant G157C. ND, not detectable.
