Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Jul 29;288(37):26678–26687. doi: 10.1074/jbc.M113.466433

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 4. — Cyclin D1 promotes the activity of NDR kinase independent of Cdk4. A, 293T cells were cotransfected with pCMV-FLAG-NDR2 alone or with pCMV-Myc-Cdk4 or pCMV-Myc-cyclin D1. The cell lysates were harvested and subjected to immunoblotting with anti-NDR phospho-Ser-281/phospho-Ser-282 antibody. B, 293T cells were cotransfected with pCMV-FLAG-NDR2 alone or with pCMV-Myc-cyclin D1 or pCMV-Myc-cyclin D1 K112E. The cell lysates were harvested and subjected to immunoblotting with anti-NDR phospho-Ser-281/phospho-Ser-282 or anti-NDR phospho-Thr-444/phospho-Thr-442 antibody. C, 293T cells were cotransfected with pCMV-FLAG-NDR2 alone or with pCMV-Myc-cyclin D1 or pCMV-Myc-cyclin D1 K112E. The cell lysates were immunoprecipitated with anti-FLAG antibody. The beads were incubated with 1 μg of GST-p21 in the presence of [γ-³²P]ATP for in vitro kinase assay. The samples were assessed by SDS-PAGE, followed by autoradiography of the gel. CBB, Coomassie Brilliant Blue.