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. 2013 Jul 29;288(37):26678–26687. doi: 10.1074/jbc.M113.466433

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© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Cyclin D1 enhances NDR activity by releasing its self-inhibition motif. A, 293T cells were cotransfected with pCMV-FLAG-NDR2 and pCMV-Myc-MOB1A with or without pCMV-Myc-cyclin D1. The cell lysates were immunoprecipitated (IP) with anti-FLAG antibody and then immunoblotted (IB) with anti-Myc antibody. B, 293T cells were cotransfected with pCMV-FLAG-NDR2 and pCMV-Myc-MST1 and with or without pCMV-Myc-cyclin D1. The cell lysates were immunoprecipitated with anti-FLAG antibody and then immunoblotted with anti-Myc antibody. C, 293T cells were transfected with pCMV-FLAG-NDR2 and pCMV-Myc-cyclin D1 with or without pCMV-Myc-MOB1A or pCMV-Myc-MST1. The cell lysates were immunoprecipitated with anti-FLAG antibody and then immunoblotted with anti-Myc antibody. D, 293T cells were transfected with the indicated plasmids. The cell lysates were harvested and subjected to immunoblotting with anti-NDR phospho-Ser-281/phospho-Ser-282 antibody or other antibodies as shown. E, 293T cells were transfected with pCMV-FLAG-NDR2 or pCMV-FLAG-NDR2 IM alone or with pCMV-Myc-cyclin D1. The cell lysates were harvested and subjected to immunoblotting with anti-NDR phospho-Ser-281/phospho-Ser-282 antibody. TCE, total cell extract.